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However, in contrast to other lactic acid bacteria, the possible beneficial effects of Weissella spp. Caenorhabditis elegans is a small, free-living soil nematode used in various fields of research. elegans is a particularly useful model to study ageing because of its short lifespan and the fact that it is amenable to genetic analyses. (b) Fluorescence was quantified using Image J software. Meanwhile, brood size was smaller in Weissella-fed worms compared with the control group (Fig. Interestingly, the reproductive periods in the Weissella-fed groups were slightly longer compared with the E. The mean total brood sizes for each pair of worms were 237, 84 and 74 in worms fed E. (b) Total brood size was determined from 30 animals, and values represent the mean for each pair of worms. coli OP50 (We next determined the level of food intake by measuring the pharyngeal pumping rate. Interestingly, many worms appeared to prefer lactic acid (125.0 ± 4.00) at 30 min after feeding on the test compounds, and again, the numbers of worms in the circle were similar (more than 300 worms) in each test at 60 min after feeding on lactic acid or 95% ethanol. 6c).(a) Depending on the degree of nuclear GFP fluorescence, three different states of translocation of DAF-16:: GFP from the cytoplasm into cell nuclei can be distinguished: cytosolic (cyt, left; no nuclear GFP fluorescence), intermediate (int, centre; weak nuclear GFP fluorescence), and nuclear (nuc, right; strong nuclear GFP fluorescence) DAF-16:: GFP localization. elegans loss-of-function mutants for each of these genes were fed Weissella or E. Weissella-fed mutant worms with loss-of-function mutations in daf-16, aak-2 or jnk-1 showed no extension in lifespan compared with the E. elegans rapidly change their behaviours and innate chemosensory preferences, ROS (by-products of respiration) are generally believed to be harmful to biological processes. Although the factor(s) involved in elevating ROS levels in W. elegans remain unknown, several positive effects have been reported regarding ROS and longevity in C. First, ROS induces the expression of superoxide dismutase (SOD), which detoxifies ROS. koreensis increased and decreased, respectively, compared with the worms fed with E. The insulin/IGF-1 receptor, DAF-2, was not related to the lifespan extension of C. It is possible that similar mechanisms of pro-longevity exist in C. Additionally, dietary restriction is known to extend lifespan and to retard age-related health declines in a number of different species, including rodents, worms, yeast and possibly primates. cibaria in de Man, Rogosa and Sharpe (MRS) broth (Difco) for 24 h without shaking.

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